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Wednesday, July 22 • 5:00pm - 5:30pm
W2 S22: Decoding cytosolic and mitochondrial calcium dynamics in astrocytes

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We discovered that the transient opening of the mitochondrial permeability transition pore induces spatially restricted Ca2+ transients in astrocytes processes, providing a means to link astrocyte respiration rates and Ca2+ dependent effector pathways. However, the cross-talk between cytosolic and mitochondrial Ca2+ signals in astrocytes still remains elusive. To simultaneously record and characterize cytosolic and mitochondrial Ca2+ dynamics, we have developed novel transgenic mouse lines and AAV based viral approaches to express various fluorescent proteins as well as genetically encoded Ca2+ indicator in various astrocytic compartments. Additionally, to automatically segment mitochondria and study the structural and Ca2+dynamics, we developed a machine-learning-based algorithm called mito-CaSCaDe. Using 2-photon microscopy-based Ca2+ imaging, we found that mitochondria exhibit spontaneous fluctuations in matrix Ca2+ and the bath application of neuromodulators induced long-lasting Ca2+ transients in mitochondria. In this workshop, I will discuss our new results that are helping us to decipher the role of astrocyte Ca2+ signals in the cytosol and mitochondria in shaping astrocyte functions in the brain.


Wednesday July 22, 2020 5:00pm - 5:30pm CEST
Crowdcast (W02)