Loading…
CNS*2020 Online has ended
Welcome to the Sched instance for CNS*2020 Online! Please read the instruction document on detailed information on CNS*2020.
Back To Schedule
Wednesday, July 22 • 5:00pm - 5:30pm
W2 S22: Decoding cytosolic and mitochondrial calcium dynamics in astrocytes

Log in to save this to your schedule, view media, leave feedback and see who's attending!

Feedback form is now closed.


We discovered that the transient opening of the mitochondrial permeability transition pore induces spatially restricted Ca2+ transients in astrocytes processes, providing a means to link astrocyte respiration rates and Ca2+ dependent effector pathways. However, the cross-talk between cytosolic and mitochondrial Ca2+ signals in astrocytes still remains elusive. To simultaneously record and characterize cytosolic and mitochondrial Ca2+ dynamics, we have developed novel transgenic mouse lines and AAV based viral approaches to express various fluorescent proteins as well as genetically encoded Ca2+ indicator in various astrocytic compartments. Additionally, to automatically segment mitochondria and study the structural and Ca2+dynamics, we developed a machine-learning-based algorithm called mito-CaSCaDe. Using 2-photon microscopy-based Ca2+ imaging, we found that mitochondria exhibit spontaneous fluctuations in matrix Ca2+ and the bath application of neuromodulators induced long-lasting Ca2+ transients in mitochondria. In this workshop, I will discuss our new results that are helping us to decipher the role of astrocyte Ca2+ signals in the cytosol and mitochondria in shaping astrocyte functions in the brain.


Speakers

Wednesday July 22, 2020 5:00pm - 5:30pm CEST
Crowdcast (W02)