Loading…
CNS*2020 Online has ended
Welcome to the Sched instance for CNS*2020 Online! Please read the instruction document on detailed information on CNS*2020.
Back To Schedule
Wednesday, July 22 • 11:30am - 12:00pm
W2 S14: Antipodes of calcium signalling: subcellular microdomains and whole cell calcium spikes

Log in to save this to your schedule, view media, leave feedback and see who's attending!

Feedback form is now closed.


There is now compelling evidence that subcellular signalling microdomains are crucial for encoding and relaying calcium (Ca2+) signals. The STIM-Orai system constitutes a critical illustration of this concept. Upon depletion of the endoplasmic reticulum (ER), both proteins move to so-called ER-PM junctions, where the plasma membrane (PM) closely apposes the ER membrane with a separation of approximately 15nm. I will present the first three-dimensional model of such ER-PM junctions and will show how the spatial organisation of Orai channels in the PM and Ca2+ pumps in the ER membrane shape the local Ca2+ signature in a non-trivial manner, which has direct consequences for downstream Ca2+ signalling. The coordination of Ca2+ increases in such microdomains can lead to whole cell Ca2+ oscillations. Given their intrinsic stochasticity, we have pursued a statistical analysis of Ca2+ spikes using concepts from stochastic point processes and Bayesian inference. I will show how we can quantify Ca2+ spiking at the single cell level in the presence of dynamic stimulation. This will not only move us closer to characterizing the role of cellular heterogeneity in Ca2+ signalling, but will also highlight how we can use whole-cell Ca2+ signals to infer properties of the subcellular Ca2+ signalling toolkit.

Speakers

Wednesday July 22, 2020 11:30am - 12:00pm CEST
Crowdcast (W02)