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Monday, July 20 • 8:00pm - 9:00pm
P7: Functional dissection of prefrontal networks using optogenetics and large-scale multi-electrode recordings

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Link to Google Meet: meet.google.com/atu-udho-ugx

Eloise V Giraud
, Jean-Philippe Thivierge, Michael Lynn, Jean-Claude Beique

The prefrontal cortex (PFC) plays an important role in executive functions that guide reward-seeking, goal-directed and memory-guided behaviours. However, the contribution of specific cell types to the activity of broader cortical circuits remains largely unknown. This is important to inform accurate computational models of prefrontal cortex function. Here, we used high-density multielectrode arrays containing 4,096 closely spaced electrodes to monitor the spiking activity of PFC neurons in acute slice preparations. We developed spike sorting techniques that combined spline interpolation and principal component analysis to distinguish regular-spiking excitatory neurons from fast-spiking inhibitory interneurons. Our sorting algorithm was validated using a targeted combination of viral and optogenetic strategies. By cell- type-specific optogenetic stimulation, we described how parvalbumin interneurons regulate the interplay between excitation and inhibition. Specifically, we characterized the influence of parvalbumin interneurons on network-wide firing rates and distance-dependent pairwise correlations within the PFC. These results form a key target for computational models that aim to capture the interactions between excitation and inhibition in cortical areas.

avatar for Eloise V Giraud

Eloise V Giraud

PhD Candidate, School of Psychology, University of Ottawa
PhD StudentUniversity of OttawaI am a PhD student working with Dr. Jean-Philippe Thivierge and Dr. Jean-Claude Béïque at the University of Ottawa. I am interested in how different cell types contribute to overall network activity in the Prefrontal Cortex. I am investigating this... Read More →

Monday July 20, 2020 8:00pm - 9:00pm CEST
Slot 11